Vyhledávat v databázi titulů je možné dle ISBN, ISSN, EAN, č. ČNB, OCLC či vlastního identifikátoru. Vyhledávat lze i v databázi autorů dle id autority či jména.
Projekt ObalkyKnih.cz sdružuje různé zdroje informací o knížkách do jedné, snadno použitelné webové služby. Naše databáze v tuto chvíli obsahuje 3349082 obálek a 1013678 obsahů českých a zahraničních publikací. Naše API využívá většina knihoven v ČR.
Development of biotechnological tools for studying infectious pathways of canine and human parvoviruses
Autor: Gilbert Leona
Rok: 2005
ISBN: 9789513921347
OKCZID: 129552509
Anotace
Parvoviruses are among the smallest vertebrate DNA viruses known to date. The production of parvovirus-like particles (parvo-VLPs) has been successfully exploited for parvovirus vaccine development. The baculovirus expression vector system (BEVS) has been the popular choice to correctly express and produce VLPs. These multimeric structures are morphologically and structurally identical to the original virus. The capsid proteins can also be produced individually or in combination to monitor structural protein functions in the parvovirus life cycle. Moreover, the ability of baculoviruses to transduce a wide range of mammalian cells has brought about the opportunity to use these viruses as gene vectors to express individual parvoviral structural proteins of interest. The present study was aimed to address a set of problems in the field of parvovirus biology and biotechnology with the development of novel parvoviral biotechnological tools. Non-fluorescent and fluorescent fusion proteins of the structural proteins of canine and human parvovirus, as well as recombinant baculoviruses encoding these proteins were successfully generated. Enhanced green fluorescent protein was incorporated on the surface of the parvo-VLPs and these recombinant baculoviruses (rBVs) were shown to be practical when following parvoviral structural proteins in vivo. The chimeric parvo-VLPs were further scrutinized specifically with regard to their assembly capabilities, trafficking events and nuclear targeting. In addition, a baculovirus-mediated de novo vector system was created to shed additional light on the synthesis and trafficking of individual parvoviral structural proteins in mammalian cells. Together, these results showed that the surface of parvoviruses could be modified, display a large foreign moiety and assemble correctly. Similarly recombinant baculoviruses could be used as gene transfer vectors to monitor the individual parvoviral proteins in transduced mammalian target cells. The parvovirus display and transduction techniques show promise as potential tools for various scientific applications ranging from basic virological studies, to gene therapy applications, and developments in biomedicine.
Zdroj anotace: OKCZ - ANOTACE Z WEBU